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Image Search Results
Journal: International Journal of Biological Sciences
Article Title: CXCL8 Drives MMP1 Upregulation and Promotes Metastatic Progression in Oral Cancer Through CXCR1/2-Mediated JAK1/STAT3 Activation
doi: 10.7150/ijbs.115990
Figure Lengend Snippet: CXCL8 is overexpressed in OSCC and associated with poor prognosis. (A) Venn diagram of upregulated DEGs identified from three GEO datasets: GSE13601 (n=29 OSCC, 29 normal), GSE143905 (n=5 OSCC, 5 normal), and GSE31056 (n=23 OSCC, 24 normal). (B) Hub genes ranked by interaction degree within the protein-protein interaction network (Cytoscape). (C) Heatmap showing expression levels of the top 40 upregulated DEGs in OSCC tissues from GSE74530 (n=6 OSCC, 6 normal). (D) Volcano plot of significantly altered genes in GSE74530 ; red: upregulated, blue: downregulated. (E) Differential expression of CXCL8 between OSCC and normal tissues in GSE74530 . (F) Kaplan-Meier analysis showing overall survival in HNSCC patients with high and low CXCL8 expression (UCSC Xena).
Article Snippet: The
Techniques: Expressing, Quantitative Proteomics
Journal: International Journal of Biological Sciences
Article Title: CXCL8 Drives MMP1 Upregulation and Promotes Metastatic Progression in Oral Cancer Through CXCR1/2-Mediated JAK1/STAT3 Activation
doi: 10.7150/ijbs.115990
Figure Lengend Snippet: CXCL8 expression positively correlates with OSCC cell motility. (A)-(B) Wound healing and Transwell migration assays comparing SCC9, SCC4, and HSC3 cell lines (n=4). (C) Western blot analysis of CXCL8 protein levels in the three cell lines (n=4). (D)-(G) The migration and wound closure were evaluated following CXCL8 (25-50 ng/ml) treatment in SCC4 and HSC3 cells (n=4). (H)-(I) Migration and wound healing ability of SCC4 subpopulations vs. parental SCC4 cells were assessed (n=4). (J)-(K) CXCL8 mRNA and protein expression in highly migratory SCC4 cells compared to parental cells were assessed (n=4). Results are expressed as the mean ± SD of four independent experiments. * p < 0.05 compared with the control group.
Article Snippet: The
Techniques: Expressing, Migration, Western Blot, Control
Journal: International Journal of Biological Sciences
Article Title: CXCL8 Drives MMP1 Upregulation and Promotes Metastatic Progression in Oral Cancer Through CXCR1/2-Mediated JAK1/STAT3 Activation
doi: 10.7150/ijbs.115990
Figure Lengend Snippet: CXCL8 promotes OSCC cell migration by inducing MMP1 expression. (A) TIMER2.0 analysis of correlations between CXCL8 and MMPs in HNSC (n=522). (B)-(D) CXCL8-induced MMP1 expression was examined by qPCR and Western blot analysis in SCC4 and HSC3 cells (n=4). (E) Validation of MMP1 knockdown efficiency by Western blot (n=4). (F)-(I) Functional analysis confirmed the effect of MMP1 knockdown on CXCL8-induced cell migration and wound healing (n=4). (J)-(K) MMP1 mRNA and protein expression of highly migratory SCC4 subpopulations were analyzed by Western blotting and qPCR (n=4). Results are expressed as the mean ± SD of four independent experiments. * p < 0.05 compared with the control group; # p < 0.05 compared with the CXCL8-treated group.
Article Snippet: The
Techniques: Migration, Expressing, Western Blot, Biomarker Discovery, Knockdown, Functional Assay, Control